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Image Search Results
Journal: Biology Open
Article Title: Sexual dimorphism in an animal model of Sjögren's syndrome: a potential role for Th17 cells
doi: 10.1242/bio.013771
Figure Lengend Snippet: Frequency of Th17 cells in salivary glands at clinical-disease stage. Glands were excised and single-cell suspensions were isolated from male and female C57BL/6 and C57BL/6J.NOD- Aec1Aec2 mice ( n =6/sex/strain, 24-33 weeks of age). Flow cytometric acquisition was performed by staining with Brilliant Violet 605 anti-mouse CD3, APC-Alexa Fluor 750 Rat anti-mouse CD4, Pacific Blue anti-mouse IFN-γ, PerCP/Cy5.5 anti-mouse IL-17A, and PE/Cy7 anti-mouse IL-4. Data was analyzed by FlowJo software. Results shown are representative data with cell count numbers presented. Average cell counts with standard of error bars for Th1, Th2, and Th17 cells of each group were presented in the bar graph (A). Immunofluorescent staining for CD4 and IL-17 were performed on paraffin-embedded sections of the salivary glands of male and female C57BL/6 and C57BL/6J.NOD- Aec1Aec2 mice ( n =6/sex/strain, 24-33 weeks of age). Negative controls were performed with rabbit IgG isotype. Representative images were presented at 200× magnification (B). Area of salivary gland Th17 cell was enumerated using binary densitometrical analysis on Nikon Elements software. (CD4 + , AF488-green; IL-17A + , AF647-pink; DAPI, blue) (B). The significance for each was calculated by two-way ANOVA. Error bars indicates s.e.m. with ** P <0.01, *** P <0.001, **** P <0.0001.
Article Snippet: In brief, slides were de-paraffinized and each section was incubated with purified
Techniques: Isolation, Staining, Software, Cell Counting
Journal: Biology Open
Article Title: Sexual dimorphism in an animal model of Sjögren's syndrome: a potential role for Th17 cells
doi: 10.1242/bio.013771
Figure Lengend Snippet: Antigen-specific hyperproliferation of Th17 cells in female C57BL/6.NOD- Aec1Aec2 . CFDA proliferation assay of salivary gland lymphocytes (A) and cervical lymph nodes (B) utilizing an antigen-non-specific manner (anti-CD3/anti-CD28), an antigen-specific activation (EC3, the third cytoplasmic loop peptide of M3R) and media alone as no stimulation for male and female C57BL/6 and C57BL/6.NOD- Aec1Aec2 . Lymphocytes were incubated with CFDA prior to stimulation conditions (4 days), followed by flow cytometry and analysis on FlowJo software. Th17 cells were gated for live CD3 + CD4 + IL-17 + . Statistically significant results were calculated with two-way ANOVA on male ( n =5) and female ( n =5) C57BL/6, and male ( n =5) and female ( n =3) C57BL/6.NOD- Aec1/2 female. Error bars indicates s.e.m. with **** P <0.00001.
Article Snippet: In brief, slides were de-paraffinized and each section was incubated with purified
Techniques: Proliferation Assay, Activation Assay, Incubation, Flow Cytometry, Software